CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes) Antibody

• Catalog Number: orb2317359
• Category: Antibodies
• Description: CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes) Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CD40LG/2763
• Tested applications: ELISA, FC, IF, IHC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Recombinant fragment of human CD40LG protein (around aa 108-261) (exact sequence is proprietary)
• Concentration: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Dilution range: ELISA (For coating, order antibody without BSA);Flow Cytometry (1-2ug/million cells);Immunofluorescence (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 36kDa
• Target: CD40LG
• Entrez: 959
• UniProt ID: P29965
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD154; gp39; hCD40L; HIGM1; IGM; IMD3; T B cell ac
• Note: For research use only
• Application notes: Positive Control: Jurkat cells. Human tonsil, spleen or thymus tissue (IHC).
• Expiration Date: 12 months from date of receipt.