CD40 Antibody

• Catalog Number: orb606499
• Category: Antibodies
• Description: CD40 is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is expressed on B-lymphocytes, follicular dendritic cells, bone marrow-derived dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zones of secondary lymphoid organs.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C40/1605
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant human protein was used as the immunogen for the CD40 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD40 antibody is available for research use only.
• UniProt ID: P25942
• Storage: Store the CD40 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD40 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of human spleen tissue with CD40 antibody (clone C40/1605). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

IHC testing of Hodgkin's Lymphoma tissue with CD40 antibody (clone C40/1605). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

IHC testing of human tonsil tissue with CD40 antibody (clone C40/1605). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

SDS-PAGE analysis of purified, BSA-free CD40 antibody (clone C40/1605) as confirmation of integrity and purity.