CD40 Antibody

• Catalog Number: orb381054
• Category: Antibodies
• Description: CD40 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IHC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived mouse CD40/TNFRSF5 recombinant protein (Position: L20-R193). Mouse CD40/TNFRSF5 shares 60.7% amino acid (aa) sequence identity with human CD40/TNFRSF5.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By HeatImmunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml ELISA , 0.1-0.5μg/mlWestern blot, 0.1-0.5μg/mlFlow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 32093 MW
• UniProt ID: P27512
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Tumor necrosis factor receptor superfamily member
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U937 cells using anti-CD40/TNFRSF5 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CD40/TNFRSF5 using anti-CD40/TNFRSF5 antibody.Lane 1:mouse spleen tissue.

IHC analysis of CD40/TNFRSF5 using anti-CD40/TNFRSF5 antibody. CD40/TNFRSF5 was detected in a paraffin-embedded section of mouse lymphaden tissue.