CD40L antibody

• Catalog Number: orb348859
• Category: Antibodies
• Description: Human monoclonal antibody to CD40L
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: hu5c8 (Ruplizumab)
• Tested applications: Blocking, FC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Human CD40L
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD40L
• UniProt ID: P29965
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD154; CD40 ligand; tumour necrosis factor ligand
• Note: For research use only
• Application notes: The antibody binds specifically to CD40L, a surface receptor expressed on activated T cells which acts as a costimulatory molecule to trigger immune responses. The antibody neutralises CD40L function, as it blocks the interaction between CD40 and CD40L. When injected into cynomolgus monkeys, the original therapeutic antibody was found to have a T1/2 of 531 ± 155 hours.
• Expiration Date: 12 months from date of receipt.

References

Karpusas, M. et al. Structure of CD40 ligand in complex with the Fab fragment of a neutralizing humanized antibody Structure, 9, 321-329 (2001)

PubMed: 11525169

IHC-P staining of human breast tumour tissue using anti-CD40L (dilution at 5 ug/ml)

Immunohistochemical staining of paraffin embedded human muscle tissue using CD40L antibody (primary antibody at 5 ug/ml)

IHC-P image of rat bladder tissue using CD40L antibody (dilution of primary antibody at 5 ug/ml)

IHC-P staining of human thyroid tumour tissue using anti-CD40L (dilution at 5 ug/ml)

Western blot analysis of rat spleen (lane 1), rat muscle (lane 2), rat bladder (lane 3), human breast tumour (lane 4), human thyroid tumour (lane 5) using CD40L antibody (1 ug/ml)