CD40 antibody

• Catalog Number: orb1152478
• Category: Antibodies
• Description: Rat monoclonal antibody to CD40
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: FGK45
• Tested applications: FA, FC, WB
• Reactivity: Mouse
• Isotype: IgG2a
• Immunogen: The original rat IgG2a antibody was raised against the recombinant mouse CD40 fusion protein.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD40
• UniProt ID: P27512
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Tnfrsf5; Tumor necrosis factor receptor superfamil
• Note: For research use only
• Application notes: FGK45 is an agonistic antibody that has been shown to activate CD40 expressing antigen presenting cells (APCs) what might promote anti-tumor T cell responses. For instance, the rat IgG2a version of this antibody was used to promote maturation of APCs what improved the ability of the macrophages to fight a tumour (Luheshi et al., 2016; pmid: 26918344). Another group used the same antibody to stimulate T-cells in a study on a tumor vaccine (Bartkowiak et al., 2015; pmid: 26351680). Others used ths clone as well to induce potent anti-tumor activity (Carmi et al., 2015; pmid: 25924063). On the other hand, it can also be used to inhibit CD40/CD154 interaction in vitro and in vivo. Furthermore, this clone can be also utilized to identify CD40-positive cells by flow cytometry (Rolink et al., 1996; pmid: 8885865). Administering this antibody can be also utilized to induce inflamatory bowel disease-like characteristics and create a mouse IBD model to study this class of diseases (Bauche et al., 2018; pmid: 30097293).
• Expiration Date: 12 months from date of receipt.