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Catalog Number | orb44808 |
---|---|
Category | Antibodies |
Description | Rat monoclonal antibody to CD4 |
Clonality | Monoclonal |
Clone Number | GK1.5 |
Tested applications | FA, FC, ICC, IHC-Fr, IP |
Reactivity | Mouse |
Isotype | Rat IgG2b |
Immunogen | Mouse CTL clone V4 cells |
Concentration | 1 mg/ml |
Dilution range | Functional application: Isolation and depletion of CD4+ T cells, blocking of ligand binding to CD4. Immunocytochemistry: Recommended dilution: 1-4 μg/ml. Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 ml lysate. Flow cytometry: Recommended dilution: 1 μg/million cells. Immunohistochemistry: Recommended dilution: 5-10 μg/ml. |
Purity | Purified by protein-G affinity chromatography. |
Conjugation | Unconjugated |
Target | CD4 |
Entrez | 12504 |
UniProt ID | P06332 |
Storage | Store at 2-8°C. Do not freeze. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4 |
Alternative names | Anti-CD 4 antibody, Anti-CD4 (L3T4) antibody, Anti Read more... |
Note | For research use only |
Application notes | Functional application: Isolation and depletion of CD4+ T cells, blocking of ligand binding to CD4. Immunocytochemistry: Recommended dilution: 1-4 μg/ml. Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 ml lysate. Flow cytometry: Recommended dilution: 1 μg/million cells. Immunohistochemistry: Recommended dilution: 5-10 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Separation of murine CD4 positive cells (red-filled) from murine CD4 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD4 (GK1.5) purified antibody (azide free, concentration in sample 4 µg/ml) DAR APC.
Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD4 (GK1.5) purified antibody (azide free, concentration in sample 4 µg/ml) DAR APC.
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