CD4 Antibody

• Catalog Number: orb1239267
• Category: Antibodies
• Description: CD4 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 9H5A8
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Mouse monoclonal CD4 antibody was raised against a 193 amino acid recombinant protein from near the amino terminus of human CD4.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-4 μg/mL; IHC: 5 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 51kDObserved: 51-57 kD (Post-modyfication: 2-4 N-linked glycosylations)
• Target: CD4
• UniProt ID: P01730
• NCBI: NP_000607
• Storage: CD4 monoclonal antibody can be stored at -20°C, stable for one year.
• Buffer/Preservatives: CD4 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: CD4 Antibody [9H5A8] : CD4mut
• Note: For research use only
• Application notes: WB: 0.5-4 μg/mL; IHC: 5 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western Blot Validation with Human Recombinant Protein.Loading: 5ng (Lane 1), 25ng (Lane 2) and 100ng (Lane 3) of human CD4 recombinant protein. Antibodies: CD4 orb1239267 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Observed at around 27kD.

Western Blot Validation in Human Thymus Tissue Lysate.Loading: 15 μg of lysates per lane. Antibodies: CD4 orb1239267 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.

Western Blot Validation in Human Jurkat Lysate.Loading: 15 μg of lysates per lane. Antibodies: CD4 orb1239267 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.

Western Blot Validation in Human, Mouse and Rat Tissue Lysate.Loading: 15 μg of lysates per lane. Antibodies: CD4 orb1239267 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.

Western Blot Validation in Human Cell Lines.Loading: 15 μg of lysates per lane. Antibodies: CD4 orb1239267 (4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.

Western Blot Validation in Mouse Spleen Tissue Lysate.Loading: 15 μg of lysates per lane. Antibodies: CD4 orb1239267 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.

Western Blot Validation in Rat Lung Tissue Lysate.Loading: 15 μg of lysates per lane. Antibodies: CD4 orb1239267 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.

Immunohistochemistry Validation of CD4 in Human Thymus Tissue.Immunohistochemical analysis of paraffin-embedded human thymus tissue using anti-CD4 antibody (orb1239267) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-mouse IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.