CD4 Antibody

• Catalog Number: orb1261848
• Category: Antibodies
• Description: CD4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-200 of human CD4 (NP_000607.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 51kDa
• Target: CD4
• UniProt ID: P01730
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: CD4 Antibody [9H5A8] : CD4mut
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of U-937 cells, using CD4 antibody at 1:500 dilution.

Immunohistochemistry of paraffin-embedded rat thymus using CD4 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse thymus using CD4 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using CD4 antibody at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using CD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using CD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of Hela cells using GRP78 / BiP antibody at dilution of 1:200. Blue: DAPI for nuclear staining.