CD4 antibody

• Catalog Number: orb44033
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD4
• Clonality: Monoclonal
• Clone Number: MEM-115
• Tested applications: FA, FC, IP
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Human thymocytes and T lymphocytes.
• Concentration: 1 mg/ml
• Dilution range: Functional application: The antibody MEM-115 blocks binding of HIV gp120 to CD4 molecule and it also strongly inhibits CD4-MHC Class II interactions. Immunoprecipitation: Excellent. Flow cytometry: Recommended dilution: 3 μg/ml. Although it has not been tested rigorously, following data suggest that the antibody MEM-115 is a low-affinity antibody: its binding to T cells increases at elevated temperature, monovalent Fab fragments essentially do not bind to T cells.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD4
• Entrez: 920
• UniProt ID: P01730
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: Anti-CD 4 antibody, Anti-CD4 (L3T4) antibody, Anti
• Note: For research use only
• Application notes: Functional application: The antibody MEM-115 blocks binding of HIV gp120 to CD4 molecule and it also strongly inhibits CD4-MHC Class II interactions. Immunoprecipitation: Excellent. Flow cytometry: Recommended dilution: 3 μg/ml. Although it has not been tested rigorously, following data suggest that the antibody MEM-115 is a low-affinity antibody: its binding to T cells increases at elevated temperature, monovalent Fab fragments essentially do not bind to T cells.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (surface staining) of human peripheral blood with anti-human CD4 (MEM-115) purified antibody (azide free), GAM-APC.