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Catalog Number | orb1289738 |
---|---|
Category | Antibodies |
Description | Rabbit Polyclonal antibody to CD4 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | Recombinant protein of human CD4. The exact sequence is proprietary. |
Dilution range | WB: 1:500-1:1000, IH: 1:50-1:200, IF:IC: 1:50-1:200 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | CD4 |
Entrez | 12504, 920, 24932 |
UniProt ID | P05540, P06332, P01730 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | T-cell surface glycoprotein CD4; T-cell surface an Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of CD4 expression in U937 (A) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 51 kD)
Immunofluorescent analysis of CD4 staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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