CD326/Epcam Antibody

• Catalog Number: orb1728194
• Category: Antibodies
• Description: CD326/Epcam Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Mouse, Rat
• Immunogen: E.coli-derived mouse CD326/Epcam recombinant protein (Position: Q24-A267).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.25 μg/ml, Mouse Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/ml, Mouse, Rat Immunofluorescence, 5 μg/ml, Mouse, Rat Flow Cytometry, 1-3 μg /1x106 cells, Mouse Direct ELISA, 0.1-0.5 μg/ml, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 38 kDa
• UniProt ID: Q99JW5
• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of RAW264.7 cells using anti-CD326/Epcam antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of CD326/Epcam using anti-CD326/Epcam antibody.Lane 1:mouse small intestine tissue;2:mouse kidney tissue.

IF analysis of CD326/Epcam using anti-CD326/Epcam antibody. CD326/Epcam was detected in a paraffin-embedded section of mouse colon cancer tissue.

IF analysis of CD326/Epcam using anti-CD326/Epcam antibody. CD326/Epcam was detected in a paraffin-embedded section of rat colon cancer tissue.

IHC analysis of CD326/Epcam using anti-CD326/Epcam antibody. CD326/Epcam was detected in a paraffin-embedded section of rat colon tissue.

IHC analysis of CD326/Epcam using anti-CD326/Epcam antibody. CD326/Epcam was detected in a paraffin-embedded section of mouse colon tissue.