EPCAM Antibody

• Catalog Number: orb1252128
• Category: Antibodies
• Description: EPCAM Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: EPM17-1
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the EpCAM antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 2-4 ug/mlIF: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Flow Cytometry: 1-2 ug/million cells in 0.1mlTitering of the EpCAM antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: EPCAM
• UniProt ID: P16422
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Epithelial cell adhesion molecule, Ep-CAM, Adenoca
• Note: For research use only
• Application notes: Western blot: 2-4 ug/mlIF: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Flow Cytometry: 1-2 ug/million cells in 0.1mlTitering of the EpCAM antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot testing of HCT116 cell lysate and EpCAM antibody (EPM17-1).

IHC testing of FFPE human colon carcinoma and EpCAM antibody (EPM17-1).

IHC testing of FFPE human colon carcinoma and EpCAM antibody (EPM17-1).

Flow cytometry testing of human MCF-7 cells using PE conjugated EpCAM antibody (red) and isotype control (green).