CD25 antibody

• Catalog Number: orb758826
• Category: Antibodies
• Description: Rat monoclonal antibody to CD25
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: PC-61.5.3
• Tested applications: ELISA, FA, FC, IF, WB
• Reactivity: Mouse
• Isotype: IgG2a
• Immunogen: This antibody was raised by immunising rats with the B6.1 mouse cytotoxic T cell line, followed by fusing spleen cells with P3X63Ag8.653 myeloma cells.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD25
• UniProt ID: P01590
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: IL2-RA; IL-2 receptor subunit alpha;L-2R alpha cha
• Note: For research use only
• Application notes: This antibody has been used in numerous FACS analyses, for instance, to demonstrate that glycolysis and glutaminolysis cooperatively control T cell function by limiting metabolite supply to N-glycosylation (Araujo et al, 2017), to suggest that IL-2C could be a potential therapeutic method to alleviate excessive inflammation and maintain blood vessel stability after traumatic brain injury (Gao et al, 2017), and to evaluate how alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells (Meininger e al, 2016). This antibody has also been used in immunofluorescence as part of the DNA Aβ42 immunization studies in mice (Lambracht-Washington et al, 2015), in ELISA assays to investigate the dynamics of T cell receptor (TCR)-dependent signaling networks (Brockmeyer et al, 2011), and in Western Blot to determine the effects of increased p300 expression on glucocorticoid receptor (GR)-T-cell-receptor (TCR) crosstalk between thymocytes (Yu et at, 2002). In addition, this antibody has been used in various in vivo functional studies, for instance, to suggest that a combination of local anti–CTLA-4 antibody production with systemic Treg depletion could enhance antitumor immune responses (Tuve et al, 2007), to provide a new insight into infection-associated tumorigenesis and illustrate the importance of antibiotic therapy to treat tumors with bacterial infiltration (Huang et al, 2007), and to demonstrate that CD4+CD25+ T cells may downregulate the development of glomerulonephritis during the preactive phase in B/WF1 mice (Hayashi et al, 2005).
• Expiration Date: 12 months from date of receipt.