CD25 antibody

• Catalog Number: orb348865
• Category: Antibodies
• Description: Human monoclonal antibody to CD25
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: Basiliximab
• Tested applications: Blocking, FC, IF, IHC, WB
• Reactivity: Human, Primate
• Isotype: IgG1
• Immunogen: Basiliximab was prepared by immunizing BALB/c mice with CTC 16 cells.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: IL-2R alpha (CD25)
• UniProt ID: P01589
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Interleukin-2 receptor subunit alpha; IL-2 recepto
• Note: For research use only
• Application notes: Basiliximab is derived from the murine anti-Tac antibody. For the clinically used format, the variable regions from this antibody were grafted onto the sequence of the human IgG1κ constant regions. Basiliximab binding to CD25 competitively blocks the interaction of IL-2R with its ligand IL-2 on the surface of T cells. Blocking this interaction prevents T cell replication and T cell-mediated B cell activation. Basiliximab binding suppresses the immune system inhibits the pathway activating the cellular immune response to allograft rejection. IL-2 signalling suppression can additionally prevent acute graft-versus-host disease, autoimmune disorders and malignancies such as T cell leukemias (Binder et al, 2007). Basiliximab has also been used in FC, IF, IHC and WB studies, which enables the detection of CD25.
• Expiration Date: 12 months from date of receipt.

References

Kovarik, J. et al. Disposition of basiliximab, an interleukin-2 receptor monoclonal antibody, in recipients of mismatched cadaver renal allografts Transplantation, 64, 1701-1705 (1997)

PubMed: 9422405

IF analysis of rat thymus tissue using anti-CD25 (dilution of primary antibody at 5 ug/ml)

Immunofluorescence analysis of rat thymus tissue using anti-CD25 (dilution of primary antibody - 5 ug/ml)

Immunohistochemical staining of paraffin embedded rat spleen tissue using CD25 antibody (primary antibody at 5 ug/ml)

Immunohistochemical staining of rat thymus tissue using CD25 antibody (dilution of primary antibody - 5 ug/ml)

IHC-P staining of pig small intestines tissue using anti-CD25 (dilution at 5 ug/ml)

Western blot analysis of human thyroid cancer (lane 1), rat spleen (lane 2), mouse lymph node (lane 3), mouse thymus (lane 4); rat small intestines (lane 5) using CD25 antibody (1 ug/ml)