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Catalog Number | orb44159 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to CD25 |
Clonality | Monoclonal |
Clone Number | MEM-181 |
Tested applications | FC |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | PHA-activated peripheral blood leucocytes |
Dilution range | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
Purity | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | APC |
Target | CD25 |
Entrez | 3559 |
UniProt ID | P01589 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | anti CD25 antibody, anti CD 25 antibody, anti IL 2 Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry analysis (surface staining) of PHA-stimulated (3 days) human PBMC with anti-CD25 (MEM-181) APC.
ICC, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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