CD25 antibody

• Catalog Number: orb44071
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD25
• Clonality: Monoclonal
• Clone Number: MEM-140
• Tested applications: FC, IP
• Reactivity: Human
• Isotype: Mouse IgM
• Immunogen: PHA-activated peripheral blood leucocytes
• Concentration: 1 mg/ml
• Dilution range: Immunoprecipitation: Excellent for immunoisolation of CD25+ cells. Flow cytometry: Recommended dilution: 1 μg/ml.
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: CD25
• Entrez: 3559
• UniProt ID: P01589
• RRID: AB_10991410
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Alternative names: anti CD25 antibody, anti CD 25 antibody, anti IL 2
• Note: For research use only
• Application notes: Immunoprecipitation: Excellent for immunoisolation of CD25+ cells. Flow cytometry: Recommended dilution: 1 μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononclear cells stained using anti-human CD25 (MEM-140) purified antibody (concentration in sample 0.6 µg/ml, GAM APC, red) and human PHA stimulated peripheral blood mononclear cells unstained by primary antibody (GAM APC, blue).