CD11b Antibody

• Catalog Number: orb1270680
• Category: Antibodies
• Description: CD11b Antibody
• Target: ITGAM
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Immunogen: This CD11b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-282 amino acids from the N-terminal region of human CD11b.
• UniProt ID: P11215
• MW: 127 kDa
• Tested applications: FC, IHC-P, WB
• Application notes: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:500For FACS starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Integrin alpha-M, CD11 antigen-like family member
• Note: For research use only
• NCBI: P11215

Western Blot at 1:2000 dilution + human spleen lysate Lysates/proteins at 20 ug per lane.

Antibody staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Antibody staining D11b in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Antibody staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:500 dilution + human spleen lysate Lysates/proteins at 20 ug per lane.

Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in A549, K562 cell line lysates (35 ug/lane).