CD11b Rabbit Polyclonal Antibody

• Catalog Number: orb11009
• Category: Antibodies
• Description: CD11b Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse CD11b (77-180/1152aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 165-170 kDa
• Target: ITGAM
• RRID: AB_10746398
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ITAM_HUMAN; Integrin alpha-M; CD11 antigen-like fa
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Liu, Qiang et al. Valproic acid attenuates inflammation of optic nerve and apoptosis of retinal ganglion cells in a rat model of optic neuritis Biomed Pharmacother, 96, 1363-1370 (2017)

PubMed: 29198746

Applications

IF, IHC

Reactivity

Rat

Elsherbiny, Nehal M. et al. Inhibitory effect of valproate sodium on pain behavior in diabetic mice involves suppression of spinal histone deacetylase 1 and inflammatory mediators Int. Immunopharmacol., 70, 16-27 (2019)

PubMed: 30785087

Applications

IHC

Reactivity

Mouse

Bai, Lijuan et al. Somatostatin prevents lipopolysaccharide-induced neurodegeneration in the rat substantia nigra by inhibiting the activation of microglia Mol Med Rep, 12, 1002-1008 (2015)

PubMed: 25777539

Applications

IHC

Reactivity

Rat

Blank control: U937 (blue). Primary Antibody: Rabbit Anti-CD11b antibody (orb11009), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb11009, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Lymph node tissue lysates, Lane 2: Mouse Spleen tissue lysates, Lane 3: Rat Spleen tissue lysates, Primary: Anti-CD11b (orb11009) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 125 kDa, Observed band size: 175 kDa.