CASP3 Antibody

• Catalog Number: orb1274467
• Category: Antibodies
• Description: CASP3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human Caspase 3 (NP_004337.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 17kDa, 35kDa
• Target: CASP3
• UniProt ID: P42574
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Caspase-3 Antibody: CPP32, SCA-1, CPP32B, CPP32, C
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of Jurkat cells, using Caspase 3 antibody (orb1274467) at 1:1000 dilution. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Western blot analysis of extracts from normal (control) and Caspase 3 knockout (KO) 293T cells, using Caspase 3 antibody (orb1274467) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Immunohistochemistry of paraffin-embedded rat spleen using Caspase 3 antibody (orb1274467) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using Caspase 3 antibody (orb1274467) at dilution of 1:100 (40x lens).