Caspase-3/CASP3 Antibody

• Catalog Number: orb215942
• Category: Antibodies
• Description: Caspase-3/CASP3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Caspase 3 recombinant protein (Position: T67-D175). Human Caspase 3 shares 86% and 90% amino acid (aa) sequences identity with mouse and rat Caspase 3, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 31608 MW
• UniProt ID: P42574
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Caspase-3;CASP-3;3.4.22.56;Apopain;Cysteine protea
• Note: For research use only
• Application notes: WB: The detection limit for Caspase 3 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of K562 cells using anti-CASP3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of K562 cells using anti-CASP3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Caspase3 using anti-Caspase3 antibody.Lane 1:human Jurkat cell;2:rat thymus tissue;3:mouse NIH/3T3 cell.

WB analysis of Caspase3 using anti-Caspase3 antibody.Lane 1:human Jurkat cell;2:human Jurkat cell;3:human Jurkat cell;4:human HepG2 cell.

IHC analysis of Caspase3 using anti-Caspase3 antibody. Caspase3 was detected in paraffin-embedded section of rat intestine tissue.

IHC analysis of Caspase3 using anti-Caspase3 antibody. Caspase3 was detected in paraffin-embedded section of human lung cancer tissue.

IHC analysis of Caspase3 using anti-Caspase3 antibody. Caspase3 was detected in paraffin-embedded section of mouse intestine tissue.