Cleaved-caspase 3 antibody

• Catalog Number: orb106556
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cleaved-caspase 3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: A synthesized peptide derived from human CASP3, corresponding to amino acid residues 163-182aa
• Dilution range: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Purity: The antiserum was purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 17kDa; 32kD(Calculated)
• Storage: Stable for 12 months from date of receipt. Store at -20 °C. Stable for 12 months from date of receipt
• Alternative names: anti Apopain antibody, anti CASP 3 antibody, anti
• Background: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Wang, Weiwei et al. Notoginsenoside R1 alleviates high glucose-evoked damage in RSC96 cells through down-regulation of miR-503 Artif Cells Nanomed Biotechnol, 47, 3947-3954 (2019)

PubMed: 31581849

Chen, Huaxia et al. Circ_0008450 downregulates Runx3 to promote the proliferation and epithelial-mesenchymal transition of human keratinized epithelial cells Cell Cycle, 19, 3303-3316 (2020)

PubMed: 33131417

Yang, Xifeng et al. Downregulation of miR‑181b inhibits human colon cancer cell proliferation by targeting CYLD and inhibiting the NF‑κB signaling pathway Int J Mol Med, 46, 1755-1764 (2020)

PubMed: 32901872

Jin, Yunfeng et al. SNAI2 promotes the development of ovarian cancer through regulating ferroptosis Bioengineered, 13, 6451-6463 (2022)

PubMed: 35220872

D'Onofrio, Nunzia et al. ROS-Mediated Apoptotic Cell Death of Human Colon Cancer LoVo Cells by Milk δ-Valerobetaine Sci Rep, 10, 8978 (2020)

PubMed: 32488123

Immunohistochemical analysis of formalin-fixed and paraffin embedded ehrlich ascites carcinoma tissue using caspase3 antibody at 1:100 dilution [Reviews]

Western blot analysis of extracts from COLO cells using Cleaved-caspase 3 antibody