Caspase 3 antibody

• Catalog Number: orb10237
• Category: Tools
• Description: Caspase 3 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human caspase-3 p17 subunit (80-175/277 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 17/32 kDa
• Target: Caspase-3
• UniProt ID: P42574
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Caspase-3 subunit p17; cleaved Caspase 3; cleaved
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Babaeenezhad, Esmaeel et al. D-Limonene Alleviates Acute Kidney Injury Following Gentamicin Administration in Rats: Role of NF-κB Pathway, Mitochondrial Apoptosis, Oxidative Stress, and PCNA Oxid Med Cell Longev, 2021, 6670007 (2021)

PubMed: 33510839

Caro-Astorga, Joaquín et al. Nascent histamine induces α-synuclein and caspase-3 on human cells Biochem Biophys Res Commun, 451, 580-586 (2014)

PubMed: 25124665

Oskoueian, Ehsan et al. Cytoprotective effect of palm kernel cake phenolics against aflatoxin B1-induced cell damage and its underlying mechanism of action BMC Complement Altern Med, 15, 392 (2015)

PubMed: 26518905

Oskoueian, Ehsan et al. Phorbol esters isolated from Jatropha meal induced apoptosis-mediated inhibition in proliferation of chang and Vero cell lines Int J Mol Sci, 13, 13816-13829 (2012)

PubMed: 23203036

Oskoueian, Ehsan et al. Phorbol esters from Jatropha meal triggered apoptosis, activated PKC-δ, caspase-3 proteins and down-regulated the proto-oncogenes in MCF-7 and HeLa cancer cell lines Molecules, 17, 10816-10830 (2012)

PubMed: 22964499

Western blot analyses for PKC-δ, Caspase-3 and GAPDH protein expression in MCF-7 cell line

Immunohistochemical analysis of paraffin-embedded rabbit heart tissue using Caspase 3 antibody

Paraffin-embedded rat brain tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). Anti Caspase 3 at 1:200 dilution with a overnight incubation period

Western blot analysis of rat brain lysates using Caspase 3 antibody