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Product Overview
Product Name Caspase 3 antibody
Catalog Number orb10237
Species/HostRabbit
ReactivityHuman, Mouse, Rat
Predicted Reactivity Bovine, Canine, Equine, Porcine, Sheep
ConjugationUnconjugated
Tested applicationsELISA, IF, IHC-P, Flow-Cyt, WB
Immunogen KLH conjugated synthetic peptide derived between 33178 amino acids of human Caspase 3
Target Caspase 3
Alternative Names
Product Properties
Form/Appearance Liquid: 0.01M TBS (pH7.4), 0.5% BSA, 0.015% ProClin™ 300 and 25% glycerol
Concentration - 5 μg (in 100 μl): 0.05 mg/ml
- 20 μg (in 100 μl): 0.2 mg/ml
- 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity Affinity purified by Protein A
MW 28 kDa
Uniprot ID P42574 (Human); P70677 (Mouse); P55213 (Rat)
NCBI NP_004337.21; NM_004346.31
Entrez 836
Product Description

Rabbit polyclonal antibody to caspase 3 is also known as apopain and whose expression can be detected in the tonsils and skeletal muscles but it is highly expressive in the spleen and kidney. It is found in the death-inducing signaling complex in plasma membrane. This protein is extensively involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a 216-Asp-|-Gly-217 bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain.

Application Notes
Dilution Range WB: 1:500-2000, ELISA: 1:500-1000, IHC-P: 1:400-800, Flow-Cyt: 2μg/Test, IF: 1:100-500
Validation Images
Western blot analyses for PKC-δ, Caspase-3 and GAPDH protein expression in MCF-7 cell line
Western blot analyses for PKC-δ, Caspase-3 and GAPDH protein expression in MCF-7 cell line
Immunohistochemical analysis of paraffin-embedded rabbit heart tissue using Caspase 3 antibody
Immunohistochemical analysis of paraffin-embedded rabbit heart tissue using Caspase 3 antibody
Paraffin-embedded rat brain tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). Anti Caspase 3 at 1:200 dilution with a overnight incubation period
Paraffin-embedded rat brain tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). Anti Caspase 3 at 1:200 dilution with a overnight incubation period
Western blot analysis of rat brain lysates using Caspase 3 antibody
Western blot analysis of rat brain lysates using Caspase 3 antibody
References

  1. Oskoueian, E. et al. Phorbol esters isolated from Jatropha meal induced apoptosis-mediated inhibition in proliferation of chang and Vero cell lines. Int J Mol Sci 13, 13816-13829 (2012)
  2. Caro-Astorga, J. et al. Nascent histamine induces α-synuclein and caspase-3 on human cells. Biochem. Biophys. Res. Commun. 451, 580-586 (2014)

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