Calbindin/CALB1 Antibody

• Catalog Number: orb182377
• Category: Antibodies
• Description: Calbindin/CALB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC, WB
• Predicted Reactivity: Bovine, Equine
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Calbindin recombinant protein (Position: A2-E175). Human Calbindin shares 99% amino acid (aa) sequence identity with both mouse and rat Calbindin.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Rat, HumanImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 30025 MW
• UniProt ID: P05937
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Calbindin;Calbindin D28;D-28K;Vitamin D-dependent
• Note: For research use only
• Application notes: WB: The detection limit for Calbindin is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-Calbindin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of HeLa cells using anti-Calbindin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Calbindin using anti-Calbindin antibody.Lane 1:Rat Brain Tissue;2:Rat Kidney Tissue.

IHC analysis of Calbindin using anti-Calbindin antibody.Calbindin was detected in paraffin-embedded section of Human Intestinal Cancer Tissue.

IHC analysis of Calbindin using anti-Calbindin antibody.Calbindin was detected in paraffin-embedded section of Human Mammary Cancer Tissue.

IHC analysis of Calbindin using anti-Calbindin antibody.Calbindin was detected in paraffin-embedded section of Mouse Kidney Tissue.

IHC analysis of Calbindin using anti-Calbindin antibody.Calbindin was detected in paraffin-embedded section of Rat Kidney Tissue.