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Catalog Number | orb443152 |
---|---|
Category | Antibodies |
Description | Calbindin/CALB1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human Calbindin recombinant protein (Position: A2-N261). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunofluorescence, 5 μg/ml, Rat Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 28 kDa |
UniProt ID | P05937 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Calbindin; Calbindin D28; D-28K; Vitamin D-depende Read more... |
Note | For research use only |
Application notes | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of A431 cells using anti-Calbindin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of Calbindin using anti-Calbindin antibody.Lane 1:rat brain tissue;2:rat kidney tissue;3:mouse brain tissue;4:mouse kidney tissue.
IF analysis of Calbindin using anti-Calbindin antibody. Calbindin was detected in a paraffin-embedded section of rat cerebellum tissue.
IF analysis of Calbindin using anti-Calbindin antibody. Calbindin was detected in a paraffin-embedded section of rat cerebellum tissue.
IHC analysis of Calbindin using anti-Calbindin antibody.Calbindin was detected in paraffin-embedded section of rat kidney tissue.
IHC analysis of Calbindin using anti-Calbindin antibody.Calbindin was detected in paraffin-embedded section of mouse brain tissue.
IHC analysis of Calbindin using anti-Calbindin antibody.Calbindin was detected in paraffin-embedded section of mouse kidney tissue.
FC, ICC, IHC, WB | |
Bovine, Equine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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