BRG1/SMARCA4 Antibody

• Catalog Number: orb413004
• Category: Antibodies
• Description: BRG1/SMARCA4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human BRG1 recombinant protein (Position: Q555-E763).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 220 kDa
• UniProt ID: P51532
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Transcription activator BRG1; TP-dependent helicas
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-BRG1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of BRG1 using anti-BRG1 antibody.Lane 1:human HeLa cell;2:human placenta tissue;3:human MCF-7 cell;4:human A549 cell;5:human A431 cell;6:human SGC-7901 cell;7:human 22RV1 cell;8:rat small intestine tissue;9:mouse small intestine tissue.

IF analysis of BRG1 using anti-BRG1 antibody.BRG1 was detected in immunocytochemical section of U20S cell.

IF analysis of BRG1 using anti-BRG1 antibody.BRG1 was detected in immunocytochemical section of U20S cell.

IHC analysis of BRG1 using anti-BRG1 antibody.BRG1 was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of BRG1 using anti-BRG1 antibody.BRG1 was detected in paraffin-embedded section of mouse small intestine tissue.

IHC analysis of BRG1 using anti-BRG1 antibody.BRG1 was detected in paraffin-embedded section of rat small intestine tissue.