BRG1/SMARCA4 Antibody

• Catalog Number: orb1529586
• Category: Antibodies
• Description: BRG1/SMARCA4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 75 and 125
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BRG1/SMARCA4
• UniProt ID: P51532
• NCBI: NP_003063.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: SWI/SNF-related matrix-associated actin-dependent
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human BRG1/SMARCA4 by western blot.Samples: Whole cell lysate from Jurkat, K-562, HEK293T, MCF-7, and HeLa cells. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibody at 0.04 µg/ml.

Detection of mouse BRG1/SMARCA4 by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibody at 0.04 µg/ml.

Detection of mouse BRG1/SMARCA4 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 used at a dilution of 1:1, 000 (0.2µg/ml).

Detection of human BRG1/SMARCA4 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-BRG1/SMARCA4 antibody.