BRG1/SMARCA4 Antibody

• Catalog Number: orb1524012
• Category: Antibodies
• Description: BRG1/SMARCA4 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 50 and 100
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BRG1/SMARCA4
• UniProt ID: P51532
• NCBI: NP_003063.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: SWI/SNF-related matrix-associated actin-dependent
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse BRG1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (50µg) , Jurkat (50µg) , and mouse NIH 3T3 (50µg) cells. Antibodies: Affinity purified goat anti-BRG1 antibody orb1524012 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human BRG1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified goat anti-BRG1 antibody orb1524012 used for IP at 6 µg/mg lysate.

Detection of mouse BRG1 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified goat anti-BRG1 (Cat. No. orb1524012) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human BRG1 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified goat anti-BRG1 (Cat. No. orb1524012) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB