BRG1 SMARCA4 Antibody (monoclonal, 3F4)

• Catalog Number: orb547167
• Category: Antibodies
• Description: BRG1 SMARCA4 Antibody (monoclonal, 3F4)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3F4
• Tested applications: FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1
• Immunogen: E. coli-derived human BRG1 recombinant protein (Position: Q555-E763).
• Concentration: 0
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 181 kDa
• UniProt ID: P51532
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-BRG1 antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of BRG1 using anti-BRG1 antibody.Lane 1:human Raji cell;2:human K562 cell;3:human HL-60 cell;4:human Caco-2 cell;5:human HeLa cell;6:human HepG2 cell;7:rat brain tissue;8:mouse brain tissue;9:mouse lung tissue.

IHC analysis of BRG1 using anti-BRG1 antibody. BRG1 was detected in paraffin-embedded section of human lung cancer tissue.

IHC analysis of BRG1 using anti-BRG1 antibody. BRG1 was detected in paraffin-embedded section of human rectal cancer tissue.

IHC analysis of BRG1 using anti-BRG1 antibody. BRG1 was detected in paraffin-embedded section of human rectal cancer tissue.

IHC analysis of BRG1 using anti-BRG1 antibody. BRG1 was detected in paraffin-embedded section of rat intestine tissue.