Beta-tubulin Antibody

• Catalog Number: orb1294417
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Beta Tubulin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the center region.
• Dilution range: Western Blot 1:5000 - 1:10000 Immunofluorescence 1:200 – 1:500
• Conjugation: Unconjugated
• Target: Beta-tubulin
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-beta Tubulin antibody - at 1/5000 dilution, Lysates at 30 μg per lane. This blot was produced using a 10% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294417 overnight at 4°C.

Anti-beta Tubulin antibody - at 1/5000 dilution, Lysates at 30 μg per lane. This blot was produced using a 10% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294417 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294417 anti-beta Tubulin antibody (red) at 1: 600 and 4°C.

Immunofluorescent analysis. Sample: HeLa cells; Red: Beta Tubulin (orb1294417): 1-400; DAPI (blue) was used as the nuclear counter stain. Fixed: ice-cold MeOH for 5 min.