Tubulin beta 3 antibody

• Catalog Number: orb44544
• Category: Antibodies
• Description: Mouse monoclonal antibody to Tubulin beta 3
• Clonality: Monoclonal
• Clone Number: TU-20
• Tested applications: FC, ICC, IHC-P, WB
• Reactivity: Canine, Human, Mouse, Porcine, Rat
• Isotype: Mouse IgG1
• Immunogen: Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Recommended dilution: 1-2 μg/ml; positive control: porcine brain lysate; negative control: HPB-ALL human peripheral blood leukemia cell line; reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml, standard ABC technique (DAB+), pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or high temperature citrate buffer antigen retrieval; positive tissue: neuronal tissue. Immunocytochemistry: Positive material: Neuro2a mouse neuroblastoma cell line. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: betaIII-Tubulin
• Entrez: 10381
• UniProt ID: Q13509
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti-beta 3 tubulin antibody, anti-beta-4 antibody
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1-2 μg/ml; positive control: porcine brain lysate; negative control: HPB-ALL human peripheral blood leukemia cell line; reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml, standard ABC technique (DAB+), pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or high temperature citrate buffer antigen retrieval; positive tissue: neuronal tissue. Immunocytochemistry: Positive material: Neuro2a mouse neuroblastoma cell line. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

References

Sana, Jiri et al. Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival Sci Rep, 8, 2836 (2018)

PubMed: 29434344

Separation of MCF-7 cells stained using anti-betaIII-Tubulin (TU-20) purified antibody (concentration in sample 9 µg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Immunocytochemistry staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20; green; 3 µg/ml). Nuclei were stained with DAPI (blue).

Immunocytochemistry staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid. A - Microtubules decorated with neuron-specific anti-betaIII-tubulin (TU-20; red). B - Merged image of co-staining with anti-beta-tubulin (TU-06; green; cat. no. orb44535). Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).

Immunohistochemistry staining of human brain (paraffin sections) using anti-betaIII tubulin (TU-20).

Immunohistochemistry staining of betaIII tubulin (red) in tissue sections of murine brain expressing GFP in some of its neurons (green). Mouse monoclonal antibody TU-20 (anti-betaIII tubulin) was detected with goat anti-mouse IgG1 conjugated with Alexa Fluor 555.

Western blotting analysis of human betaIII-tubulin using mouse monoclonal antibody TU-20 on lysates of U87 cells and Neuro 2a cells (and Jurkat cells as a negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-betaIII-tubulin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for betaIII-tubulin at approximately 55 kDa.