Beta Catenin Antibody

• Catalog Number: orb2639979
• Category: Antibodies
• Description: Beta Catenin is a dual function protein, regulating the coordination of cell–cell adhesion and gene transcription. In humans, the CTNNB1 protein is encoded by the CTNNB1 gene. In Drosophila, the homologous protein is called armadillo. b-Catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. It is a member of the catenin protein family and homologous to gamma-Catenin, also known as plakoglobin. b-Catenin is widely expressed in many tissues. In cardiac muscle, it localizes to adherens junctions in intercalated disc structures, which are critical for electrical and mechanical coupling between adjacent cardiomyocyte. [Wiki]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 6F9
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Chicken beta-Catenin was used as the immunogen for the b-Catenin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This b-Catenin antibody is available for research use only.
• UniProt ID: P35222
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Beta Catenin antibody to be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human tonsil tissue with b-Catenin antibody (clone 6F9). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 10-20 min.

Western blot testing of human HeLa cell lysate with b-Catenin antibody (clone 6F9). Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.

SDS-PAGE Analysis of Purified, BSA-Free b-Catenin Antibody (clone 6F9). Confirmation of Integrity and Purity of the Antibody.