BACE1 antibody

• Catalog Number: orb412336
• Category: Antibodies
• Description: Rabbit polyclonal antibody to BACE1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human BACE1
• Dilution range: WB: 1:500-2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: BACE1
• Entrez: 29392, 23821, 23621
• UniProt ID: P56818, P56817, P56819
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-BACE antibody, anti-KIAA1149 antibody, anti-B
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of BACE1 expression in MCF7 (A), THP1 (B), mouse brain (C), rat heart (D) whole cell lysates. (Predicted band size: 42; 45; 48; 51; 52; 55 kD; Observed band size: 62 kD)

Immunofluorescent analysis of BACE1 staining in U251MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.