BACE1 Antibody

• Catalog Number: orb1273668
• Category: Antibodies
• Description: BACE1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3C1C3
• Tested applications: ELISA, FC, ICC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Rurified recombinant fragment of human BACE1 (AA: 112-324) expressed in E. Coli.
• Concentration: batch dependent
• Dilution range: ELISA: 1/10000Western Blot: 1/500 - 1/2000ICC: 1/200 - 1/1000Flow Cytometry: 1/200 - 1/400
• Conjugation: Unconjugated
• Target: BACE1
• UniProt ID: P56817
• Storage: BACE monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide.
• Alternative names: BACE Antibody: ASP2, BACE, HSPC104, KIAA1149, Beta
• Note: For research use only
• Application notes: ELISA: 1/10000Western Blot: 1/500 - 1/2000ICC: 1/200 - 1/1000Flow Cytometry: 1/200 - 1/400
• Expiration Date: 12 months from date of receipt.

Control Antigen (100 ng); Purple line: Antigen(10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Western blot analysis using BACE1 mAb against human BACE1 recombinant protein. (Expected MW is 49.9 kDa)

Western blot analysis using BACE1 mAb against HEK293 (1) and BACE1 -hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using BACE1 mouse mAb against Hela (1), SK-N-SH (2), HepG2 (3), C6 (4), PC-12 (5), PANC-1 (6), NIH/3T3 (7) cell lysate.

Flow cytometric analysis of Hela cells using BACE1 mouse mAb (green) and negative control (red).

Immunofluorescence analysis of MCF-7 cells using BACE1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher.