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BACE1 antibody

Catalog Number: orb411845

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb411845
CategoryAntibodies
DescriptionRabbit polyclonal antibody to BACE1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant full length protein of human BACE1
Dilution rangeWB: 1:500-2000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetBACE1
Entrez23621, 23821, 29392
UniProt IDP56819, P56818, P56817
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti-BACE antibody, anti-KIAA1149 antibody, anti-B
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NoteFor research use only
Expiration Date12 months from date of receipt.
BACE1 antibody

Western blot analysis of BACE1 expression in U251 (A), HT1080 (B) whole cell lysates. (Predicted band size: 42; 45; 48; 51; 52; 55 kD; Observed band size: 68 kD)

BACE1 antibody

Immunofluorescent analysis of BACE1 staining in U251MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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