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Catalog Number | orb411845 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to BACE1 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | Recombinant full length protein of human BACE1 |
Dilution range | WB: 1:500-2000 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | BACE1 |
Entrez | 23621, 23821, 29392 |
UniProt ID | P56819, P56818, P56817 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | anti-BACE antibody, anti-KIAA1149 antibody, anti-B Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of BACE1 expression in U251 (A), HT1080 (B) whole cell lysates. (Predicted band size: 42; 45; 48; 51; 52; 55 kD; Observed band size: 68 kD)
Immunofluorescent analysis of BACE1 staining in U251MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ELISA, ICC, IF, IHC-P, WB | |
Bovine, Guinea pig, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, PLA, WB | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Zebrafish | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-Fr, IHC-P, WB | |
Canine, Gallus, Guinea pig, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |