ANXA2 Antibody

• Catalog Number: orb1263234
• Category: Antibodies
• Description: ANXA2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Bovine, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This ANXA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-49 amino acids from the N-terminal region of human ANXA2.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 39 kDa
• Target: ANXA2
• UniProt ID: P07355
• NCBI: P07355
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Annexin A2, Annexin II, Annexin-2, Calpactin I hea
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Antibody staining ANXA2 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lane 4: SH-SY5Y whole cell lysates Lane 5: SK-BR-3 whole cell lysates Lysates/proteins at 20 ug per lane.

Western blot analysis of ANXA2 using rabbit polyclonal ANXA2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ANXA2 gene.

Western blot analysis of anti-ANXA2 Antibody in mouse lung tissue lysates (35 ug/lane).

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot analysis in 293, MDA-MB231 cell line lysates (35 ug/lane).