ZASP Antibody

• Catalog Number: orb378153
• Category: Antibodies
• Description: The ZASP Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human ZASP .Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human ZASP
• UniProt ID: O75112, Q9JKS4
• Tested applications: IF, IHC, WB
• Dilution range: WB: 1:500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KIAA0613; ZASP; LIM domain-binding protein 3; Prot
• Research Area: Cardiovascular Disease, Epigenetics
• Note: For research use only
• Entrez: 11155, 24131

Western blot analysis of ZASP expression in Hela (A), MCF7 (B), mouse skeletal muscle (C), rat skeletal muscle (D) whole cell lysates. (Predicted band size: 30; 35; 42; 50; 66; 77; 78 kD; Observed band size: 50; 90 kD)

Immunohistochemical analysis of ZASP staining in mouse stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of ZASP staining in human placenta. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).