ZASP/ CYPHER/LDB3 Antibody

• Catalog Number: orb19099
• Category: Antibodies
• Description: Goat polyclonal antibody to LDB3
• Target: ZASP/ CYPHER / LDB3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KSQNKPEDEADE
• RRID: AB_10755756
• MW: 77.1; 66.7; 35.6; 31.0
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:1000, WB: 0.1-1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Experiments gave bands at approx 80-90kDa and 30-35kDa in Human Skeletal Muscle lysates after 0.01 μg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Huang et al J Biol Chem. 2003 Feb 28;278(9):7360-5; PMID: 12499364). This protein has a calculated MW of 77.1kDa according to NP_009009.1. Recommended concentration: 0.01-0.03 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti LDB3 antibody, anti ZASP antibody, anti LIM d
• Research Area: Cardiovascular Disease, Epigenetics
• Note: For research use only
• Entrez: 11155
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human muscle lysate using LDB3 antibody

Immunohistochemical staining of Human Skeletal Muscle using LDB3 antibody