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ZASP/ CYPHER/LDB3 Antibody

SKU: orb19099

Description

Goat polyclonal antibody to LDB3

Research Area

Cardiovascular Disease, Epigenetics

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution rangeELISA: 1:1000, WB: 0.1-1 μg/ml
ReactivityHuman
Predicted ReactivityCanine, Mouse, Porcine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Experiments gave bands at approx 80-90kDa and 30-35kDa in Human Skeletal Muscle lysates after 0.01 μg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Huang et al J Biol Chem. 2003 Feb 28;278(9):7360-5; PMID: 12499364). This protein has a calculated MW of 77.1kDa according to NP_009009.1. Recommended concentration: 0.01-0.03 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetZASP/ CYPHER / LDB3
Molecular Weight77.1; 66.7; 35.6; 31.0
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti LDB3 antibody, anti ZASP antibody, anti LIM domain binding 3 antibody, anti HGNC:15710 antibody, anti KIAA01613 antibody, anti KIAA0613 antibody, anti ORACLE antibody, anti PDLIM6 antibody, anti PDZ and LIM domain 6 antibody, anti Z-band alternatively spliced PDZ-motif antibody, anti CYPHER antibody, anti FLJ35865 antibody, anti ldb3z1 antibody, anti ldb3z4 antibody
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ZASP/ CYPHER/LDB3 Antibody

Western blot analysis of human muscle lysate using LDB3 antibody

ZASP/ CYPHER/LDB3 Antibody

Immunohistochemical staining of Human Skeletal Muscle using LDB3 antibody

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

ZASP/ CYPHER/LDB3 Antibody (orb19099)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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