Anti-HIF1 alpha Antibody

• Catalog Number: orb1423078
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HIF1 alpha.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: HIF1A
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: WB: WB (1/500 - 1/1000), IH (1/100 - 1/200)
• Conjugation: Unconjugated
• UniProt ID: Q16665
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of HIF1 alpha staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of HIF1 alpha expression in HEK293T (A), A549 (B), MCF7 (C), H9C2 (D), SP20 (E) whole cell lysates.