Anti-HIF1 alpha Antibody

• Catalog Number: orb216126
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HIF1 alpha
• Target: HIF1A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HIF1 alpha. The exact sequence is proprietary.
• UniProt ID: O35800, Q61221, Q16665
• Tested applications: IF, IH, WB
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1-200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-ARNT interacting protein antibody, anti-HIF 1
• Note: For research use only
• Entrez: 29560, 15251, 3091

Western blot analysis of HIF1 alpha expression in HEK293T (A), A549 (B), MCF7 (C), H9C2 (D), SP20 (E) whole cell lysates. (Predicted band size: 92 kD; Observed band size: 120; 93 kD)

Immunohistochemical analysis of HIF1 alpha staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of HIF1 alpha staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).