Rabbit HIF1-alpha Antibody

SKU: orb1530414

Description

HIF1-alpha Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: ICC, IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 for 20 minutes using a pressure cooker is recommended for FFPE tissue sections.; ICC-IF - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes using a pressure cooker is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Gallus, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 776 and C-terminus
• Target: HIF1-alpha
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ARNT-interacting protein; basic-helix-loop-helix-PAS protein MOP1; bHLHe78; class E basic helix-loop-helix protein 78; HIF1; HIF-1A; HIF-1alpha; HIF-1-alpha; HIF1-ALPHA; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8

Detection of mouse HIF1 alpha by western blot.Samples: NIH3T33ells that were either treated with 200 µM cobalt chloride (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody orb1530414 at 0.04 µg/ml.

Detection of human HIF1 alpha by western blot.Samples: HepG2 cells that were either treated with 200 µM cobalt chloride (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody orb1530414 at 0.04 µg/ml.

Detection of human HIF1-alpha by immunohistochemistry. Sample: FFPE section of renal cell carcinoma. Antibody: Affinity purified rabbit anti-HIF1-alpha antibody used at a dilution of 1:100 (2µg/ml).

Detection of human HIF1 alpha by western blot of immunoprecipitates.Samples: Whole cell lysate from HepG2 cells that were treated with 200 µM cobalt chloride or untreated. Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody.

ICC: Sample: HeLa cells. Antibody: Rabbit anti-HIF1-alpha antibody. Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated. Counterstain: DAPI (blue) and fluorescent phalloidin (green).

Quick Database Links

Gene Symbol

HIF1-alpha

UniProt

Q16665

RefSeq (Protein):NP_851397.1