Rabbit anti-HIF1-alpha Antibody

• Catalog Number: orb1530414
• Category: Antibodies
• Description: HIF1-alpha Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 776 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 for 20 minutes using a pressure cooker is recommended for FFPE tissue sections.; ICC-IF - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes using a pressure cooker is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: HIF1-alpha
• UniProt ID: Q16665
• NCBI: NP_001521.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: HIF1; hypoxia inducible factor 1, alpha subunit (b
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse HIF1 alpha by western blot.Samples: NIH3T33ells that were either treated with 200 µM cobalt chloride (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody orb1530414 at 0.04 µg/ml.

Detection of human HIF1 alpha by western blot.Samples: HepG2 cells that were either treated with 200 µM cobalt chloride (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody orb1530414 at 0.04 µg/ml.

Detection of human HIF1-alpha by immunohistochemistry. Sample: FFPE section of renal cell carcinoma. Antibody: Affinity purified rabbit anti-HIF1-alpha antibody used at a dilution of 1:100 (2µg/ml).

Detection of human HIF1 alpha by western blot of immunoprecipitates.Samples: Whole cell lysate from HepG2 cells that were treated with 200 µM cobalt chloride or untreated. Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody.

ICC: Sample: HeLa cells. Antibody: Rabbit anti-HIF1-alpha antibody. Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated. Counterstain: DAPI (blue) and fluorescent phalloidin (green).