Anti-Frizzled 5 Antibody

• Catalog Number: orb737324
• Category: Antibodies
• Description: Rabbit polyclonal antibody to FZD5
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: FZD5; FZD8
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: WB: 1/500 - 1/1000, IF: 1/50 - 1/200
• Conjugation: Unconjugated
• UniProt ID: Q13467
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-FZD5 antibody, anti-C2orf31 antibody, anti-Fr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Frizzled 5 expression in SGC7901 (A), PC3 (B), MEF (C) whole cell lysates.

Immunofluorescent analysis of Frizzled 5 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.