Androgen Receptor Antibody

• Catalog Number: orb1523846
• Category: Antibodies
• Description: Androgen Receptor Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 1 and 50
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:20 to 1:80. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Androgen Receptor
• UniProt ID: P10275
• NCBI: NP_000035.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: HYSP1; NR3C4; TFM; SMAX1; nuclear receptor subfami
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Androgen Receptor by western blot. Samples: Whole cell lysate (50 µg) from LNCaP and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Androgen Receptor antibody (orb1523846) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human Androgen Receptor by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from LNCaP cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Androgen Receptor antibody (orb1523846) used for IP at 6 µg per reaction. Androgen Receptor was also immunoprecipitated by a previous lot of this antibody (orb1523846) and a second antibody against a different epitiope of Androgen Receptor.

Detection of human Androgen Receptor by immunohistochemistry.Sample: FFPE section of human testiclar seminoma. Antibody: Affinity purified rabbit anti-Androgen Receptor (orb1523846) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB