AR Antibody

• Catalog Number: orb1248019
• Category: Antibodies
• Description: AR Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ChIP, ELISA, IF, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: EVQLGLGRVYPRPPSC
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 100kDa band observed in Human Cerebellum lysates (calculated MW of 99.2kDa according to NP_000035.2). Recommended concentration:0.1-0.5ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunohistochemistry:In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3ug/ml.Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10ug/ml. Chromatin Immunoprecipitation: A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using EB06441 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: AR
• UniProt ID: P10275
• NCBI: NP_000035.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: AR, androgen receptor, dihydrotestosterone recepto
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 100kDa band observed in Human Cerebellum lysates (calculated MW of 99.2kDa according to NP_000035.2). Recommended concentration:0.1-0.5ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunohistochemistry:In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3ug/ml.Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10ug/ml. Chromatin Immunoprecipitation: A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using EB06441 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.
• Expiration Date: 12 months from date of receipt.

orb1248019 (0.5 ug/ml) staining of Human Cerebellum (A) and (0.1 ug/ml) negative control Pancreas (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248019 (2 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

ChIP of 2 ug orb1248019 with 1 ug DHT-treated HEC50 chromatin using the Chromatrap® spin column sonication kit (Protein G) measuring FKBP5 enrichment.

orb1248019 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Mitochondrial/cytoplasmic staining.

orb1248019 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Mitochondrial/cytoplasmic staining.