Ran Antibody

• Catalog Number: orb402333
• Category: Antibodies
• Description: Ran Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Ran recombinant protein (Position: A2-L216). Human Ran shares 100% amino acid (aa) sequence identity with both mouse and rat Ran.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat, Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 24423 MW
• UniProt ID: P62826
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: GTP-binding nuclear protein Ran;Androgen receptor-
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-Ran antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U937 cells using anti-Ran antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Ran using anti-Ran antibody.Lane 1:rat brain Tissue;2:rat testis Tissue;3:rat thymus Tissue;4:mouse brain Tissue;5:mouse testis Tissue;6:mouse thymus Tissue;7:human A549 Cell;8:human 22RV1 Cell;9:human HeLa Cell.

IF analysis of Ran using anti-Ran antibody.Ran was detected in immunocytochemical section of U20S cell.

IHC analysis of Ran using anti-Ran antibody.Ran was detected in paraffin-embedded section of mouse small intestine tissue.

IHC analysis of Ran using anti-Ran antibody.Ran was detected in paraffin-embedded section of rat small intestine tissue.

IHC analysis of Ran using anti-Ran antibody.Ran was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of Ran using anti-Ran antibody.Ran was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of Ran using anti-Ran antibody.Ran was detected in paraffin-embedded section of human lung cancer tissue.