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AMACR / p504S Antibody (Prostate Cancer Marker)

Catalog Number: orb2642638

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2642638
CategoryAntibodies
DescriptionThis antibody recognizes a protein of 43kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and -oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.
Species/HostMouse
ClonalityMonoclonal
Clone NumberAMACR/1864
Tested applicationsIHC-P, WB
ReactivityHuman
IsotypeMouse IgG1, kappa
ImmunogenA portion of amino acids 297-394 was used as the immunogen for the AMACR antibody.
Antibody TypePrimary Antibody
Dilution rangeWestern blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis AMACR antibody is available for research use only.
UniProt IDQ9UHK6
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the AMACR antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
AMACR / p504S Antibody (Prostate Cancer Marker)

IHC testing of FFPE human renal carcinoma with AMACR antibody. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

AMACR / p504S Antibody (Prostate Cancer Marker)

IHC testing of FFPE human prostate carcinoma with AMACR antibody. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

AMACR / p504S Antibody (Prostate Cancer Marker)

Western blot testing of human PC-3 cell lysate with AMACR antibody. Predicted molecular weight ~43 kDa.

AMACR / p504S Antibody (Prostate Cancer Marker)

Western blot testing of 1) human partial protein and 2) human kidney lysate with AMACR antibody. Predicted molecular weight ~43 kDa.

AMACR / p504S Antibody (Prostate Cancer Marker)

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using AMACR antibody (clone AMACR/1864). These results demonstrate the foremost specificity of the AMACR/1864 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

AMACR / p504S Antibody (Prostate Cancer Marker)

SDS-PAGE analysis of purified, BSA-free AMACR antibody (clone AMACR/1864) as confirmation of integrity and purity.

AMACR / p504S Antibody (Prostate Cancer Marker)

Western blot testing of human kidney lysate with AMACR antibody. Predicted molecular weight ~43 kDa.

  • AMACR / p504S Antibody (Prostate Cancer Marker) [orb606943]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • AMACR / p504S Antibody (Prostate Cancer Marker) [orb2638392]

    IHC-P,  WB

    Human

    Rabbit

    Monoclonal

    Unconjugated

    0.1 ml
  • AMACR / p504S Antibody (Prostate Cancer Marker) [orb385979]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • AMACR / p504S Antibody (Prostate Cancer Marker) [orb316989]

    IHC-P,  WB

    Human

    Rabbit

    Monoclonal

    Unconjugated

    0.5 ml
  • AMACR / p504S Antibody (Prostate Cancer Marker) [orb2639955]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg