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AMACR / p504S Antibody (Prostate Cancer Marker)

Catalog Number: orb2639955

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2639955
CategoryAntibodies
DescriptionAMACR, also known as p504S is an enzyme that is involved in bile acid biosynthesis and beta-oxidation of branched-chain fatty acids. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands.
Species/HostMouse
ClonalityMonoclonal
Clone NumberAMACR/1723
Tested applicationsIHC-P, WB
ReactivityHuman
IsotypeMouse IgG2b, lambda
ImmunogenA recombinant human protein was used as the immunogen for the AMACR antibody.
Antibody TypePrimary Antibody
Dilution rangeWestern blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis AMACR antibody is available for research use only.
UniProt IDQ9UHK6
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesThe stated application concentrations are suggested starting amounts. Titration of the Gamma Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Expiration Date12 months from date of receipt.
AMACR / p504S Antibody (Prostate Cancer Marker)

Western blot testing of human PC3 cell lysate with AMACR antibody. Predicted molecular weight ~43 kDa.

AMACR / p504S Antibody (Prostate Cancer Marker)

Western blot testing of human kidney lysate with AMACR antibody. Predicted molecular weight ~43 kDa.

AMACR / p504S Antibody (Prostate Cancer Marker)

Western blot testing of human liver lysate with AMACR antibody. Predicted molecular weight ~43 kDa.

AMACR / p504S Antibody (Prostate Cancer Marker)

IHC testing of FFPE human prostate carcinoma with AMACR antibody (clone AMACR/1723). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

AMACR / p504S Antibody (Prostate Cancer Marker)

SDS-PAGE Analysis of Purified, BSA-Free AMACR Antibody (clone AMACR/1723). Confirmation of Integrity and Purity of the Antibody.

  • AMACR / p504S Antibody (Prostate Cancer Marker) [orb606943]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • AMACR / p504S Antibody (Prostate Cancer Marker) [orb2642638]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • AMACR / p504S Antibody (Prostate Cancer Marker) [orb2638392]

    IHC-P,  WB

    Human

    Rabbit

    Monoclonal

    Unconjugated

    0.1 ml
  • AMACR / p504S Antibody (Prostate Cancer Marker) [orb385979]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • AMACR / p504S Antibody (Prostate Cancer Marker) [orb316989]

    IHC-P,  WB

    Human

    Rabbit

    Monoclonal

    Unconjugated

    0.5 ml