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AMACR / p504S Antibody (Prostate Cancer Marker)

Catalog Number: orb2638392

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb2638392
CategoryAntibodies
DescriptionThis antibody recognizes a protein of 44kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and beta-oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.
ClonalityMonoclonal
Species/HostRabbit
IsotypeRabbit IgG
ConjugationUnconjugated
ReactivityHuman
Buffer/PreservativesBioreactor concentrate with 0.05% sodium azide
PurityUnpurified high titer supernatant
ImmunogenFull length human recombinant protein was used as the immunogen for the p504S antibody.
UniProt IDQ9UHK6
Tested applicationsIHC-P, WB
Dilution rangeImmunohistochemistry (FFPE): 1:50-1:100 for 30-60 min at RT,Western blot: 1:-50-1:100
Application notesOptimal dilution of the p504S antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Antibody TypePrimary Antibody
Clone Number13H4
FormulaBioreactor concentrate with 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis p504S antibody is available for research use only.
NoteFor research use only
AMACR / p504S Antibody (Prostate Cancer Marker)

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with p504S antibody (13H4)

AMACR / p504S Antibody (Prostate Cancer Marker)

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with p504S antibody (13H4)

AMACR / p504S Antibody (Prostate Cancer Marker)

IHC staining of FFPE human prostate carcinoma with p504S antibody (clone 13H4). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

AMACR / p504S Antibody (Prostate Cancer Marker)

IHC staining of FFPE human renal cell carcinoma with p504S antibody (clone 13H4). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

AMACR / p504S Antibody (Prostate Cancer Marker)

Western blot testing of human kidney lysate with AMACR antibody. Predicted molecular weight ~43 kDa.

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    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
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    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg
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    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
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    IHC-P,  WB

    Human

    Rabbit

    Monoclonal

    Unconjugated

    0.5 ml
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    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg