AMACR Antibody (Prostate Cancer Marker)

SKU: orb385979

Description

AMACR, also known as p504S is an enzyme that is involved in bile acid biosynthesis and beta-oxidation of branched-chain fatty acids. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the AMACR antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, lambda
• Clone No.: AMACR/1723
• Immunogen: A recombinant human protein was used as the immunogen for the AMACR antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human PC3 cell lysate with AMACR antibody. Predicted molecular weight ~43 kDa.

Western blot testing of human kidney lysate with AMACR antibody. Predicted molecular weight ~43 kDa.

Western blot testing of human liver lysate with AMACR antibody. Predicted molecular weight ~43 kDa.

IHC testing of FFPE human prostate carcinoma with AMACR antibody (clone AMACR/1723). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE Analysis of Purified, BSA-Free AMACR Antibody (clone AMACR/1723). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

Q9UHK6