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Catalog Number | orb385979 |
---|---|
Category | Antibodies |
Description | AMACR, also known as p504S is an enzyme that is involved in bile acid biosynthesis and beta-oxidation of branched-chain fatty acids. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | AMACR/1723 |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG2b, lambda |
Immunogen | A recombinant human protein was used as the immunogen for the AMACR antibody. |
Antibody Type | Primary Antibody |
Dilution range | Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This AMACR antibody is available for research use only. |
UniProt ID | Q9UHK6 |
Storage | Store the AMACR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the AMACR antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
Western blot testing of human PC3 cell lysate with AMACR antibody. Predicted molecular weight ~43 kDa.
Western blot testing of human kidney lysate with AMACR antibody. Predicted molecular weight ~43 kDa.
Western blot testing of human liver lysate with AMACR antibody. Predicted molecular weight ~43 kDa.
IHC testing of FFPE human prostate carcinoma with AMACR antibody (clone AMACR/1723). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE Analysis of Purified, BSA-Free AMACR Antibody (clone AMACR/1723). Confirmation of Integrity and Purity of the Antibody.
IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |