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Catalog Number | orb1929628 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB22732 |
Tested applications | FC, IF, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 55769 Da |
Target | This AKT2 antibody is generated from rabbits immunized with human AKT2 recombinant protein. |
UniProt ID | P31751 |
NCBI | NP_001617.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | RAC-beta serine/threonine-protein kinase, Protein Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
AKT2 Antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of AKT2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of AKT2 Antibody in NCI-H460 cell line lysates (35 ug/lane). AKT2 (arrow) was detected using the purified Pab.
Western blot analysis of AKT2 Antibody in mouse brain tissue lysates (35 ug/lane). AKT2 (arrow) was detected using the purified Pab.
FC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Gallus, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Gallus, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |