AKT2 Antibody

• Catalog Number: orb182375
• Category: Antibodies
• Description: AKT2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human AKT2 (454-481aa DRYDSLGLLELDQRTHFPQFSYSASIRE), different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, -Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 55769 MW
• UniProt ID: P31751
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: RAC-beta serine/threonine-protein kinase;2.7.11.1;
• Note: For research use only
• Application notes: WB: The detection limit for AKT2 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of AKT2 using anti-AKT2 antibody.Lane 1:HELA Cell;2:PANC Cell;3:A549 Cell;4:COLO320 Cell;5:HT1080 Cell;6:MCF-7 Cell.

IHC analysis of AKT2 using anti-AKT2 antibody.AKT2 was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of AKT2 using anti-AKT2 antibody.AKT2 was detected in paraffin-embedded section of human lung cancer tissues.

IHC analysis of AKT2 using anti-AKT2 antibody.AKT2 was detected in frozen section of rat brain tissues.